Posters

Evaluation of pharmacogenetic markers by exome-sequencing of DNA extracted from saliva samples (pdf)

With the rapid technological advancement and decreasing cost of next generation sequencing that has occurred over the last few years, it has become more practical to sequence larger numbers of samples.

Evaluation of oral samples and tracheal fluid collection from premature newborns as a source of DNA for genotyping (pdf)

Newborn screening is an important procedure in which newborn infants are screened for a list of genetic diseases. Recently, it has been suggested that screening dried blood spots for some infections may not be suitable and that other specimen types, such as oral samples and tracheal fluids, should be explored.

Rapid screening of genetic factors associated with warfarin dosage using a nanofluidic system (pdf)

There is a need for systems allowing medium multiplexing with high throughput excellent call rates, high concordance and low cost. We demonstrate the use of such a system, using DNA samples extracted from saliva.

Evaluation of a new commercial kit for detection of human herpesvirus 8 (HHV-8) in saliva. (pdf)

DNA Genotek offers a commercial salivary collection kit useful for examining human genomic DNA, but are expanding into microbial diagnostics. Our study evaluated the DNA Genotek prototype kit (P-021) designed to stabilize microbial DNA, in both invitro spiking studies and a Kenyan field trial to compare the DNA Genotek kit against traditional methods to quantitate HHV-8 oral shedding in HIV-positive patients in Kenya and determine HHV-8 subtypes.

Saliva samples collected and stabilized with Oragene®•DNA are a reliable source of DNA for next generation sequencing (pdf)

Saliva is a reliable source of DNA for use with the Agilent SureSelect Human All Exon Kit and sequencing using paired-end methodology on the Illumina Genome Analyzer II as demonstrated by the coverage (98.7%), depth (74x), and genome aligment (97.3%).

Enabling genome discovery in sheep: Superior DNA collection from nasal samples using Performagene•LIVESTOCK (pdf)

Nasal sample collection is an easy-to-use and reliable alternative that provides high quantity, high quality genomic DNA from sheep.

Development of a PCR-assay to detect papillomavirus in the snow leopard (pdf)

The genome sequence of PV1 from the snow leopard was used to develop a PCR assay to detect viral DNA. DNA samples were collected with Oragene•ANIMAL.

Evaluation of the performance of gDNA from saliva collected with Oragene•DNA for the purpose of CNV analysis on the Agilent Human Genome CGH Array 244A (pdf)

This poster presents the results of a study that evaluates the performance of gDNA from saliva on the Agilent Human Genome CGH Array 244K.

Evaluation of the performance of genomic DNA from saliva collected with Oragene•DNA for the purpose of SNP discovery on various Illumina technologies (pdf)

The use of genomic DNA extracted from saliva collected using Oragene•DNA self-collection kits for SNP and CNV analysis is evaluated on Illumina BeadChip technologies. The performance of paired blood and saliva samples are compared and the intra-donor reproducibility of the results is demonstrated.

Saliva specimens collected with Oragene are a reliable alternative to blood and buccal swabs for large scale DNA extraction and HLA typing of recruits for Hematopoietic stem cell donor registries (pdf)

Anthony Nolan Trust investigated the move from blood to self collection specimens for molecular HLA typing of volunteers wishing to join the registry. Scientific publications and personal recommendations led to a belief that an Oragene system would be amenable to high throughput automated DNA extraction with yield and quality acceptable for molecular typing requirements.